The Methods Library
Explore experimental models and analytical approaches, with their applications, measurements and published validation evidence.
A fluorescence-supervised image-analysis framework that estimates viability, spatial cell-death patterns and morphology from bright-field images of individual spheroids and organoids.
Survival, lipid, mitochondrial, expression and calcium-imaging readouts compare semaglutide responses in forebrain organoids at different maturation stages.
Organoid-derived proximal tubule epithelia provide bidirectional drug-transport and inhibitor-response measurements for OAT1/3, OCT2/MATE and related transport pathways.
A PFPE flow path reduces device sorption while separate intestinal and hepatic dosing measurements estimate the contributions to midazolam availability.
A flexible microelectrode array (MEA) and scoop-shaped well combine extracellular recording and video to compare cardiac organoids from healthy donors and patients with Duchenne muscular dystrophy (DMD).
Organoids and primary cells obtained from the same tumor provide paired material for structural and molecular comparisons, with an exploratory two-patient temozolomide experiment.
Organoid cell dynamics, physiologically based pharmacokinetic (PBPK) exposure estimates and epithelial turnover are integrated to examine clinical diarrhea incidence and severity.
A human cortical organoid system combines morphology, gene expression, cell composition and neuronal network measurements after brief exposure to the parent drug.
A two-compartment chip enables separate sampling on vascular and cerebrospinal-fluid sides while measuring barrier permeability, PEG distribution and drug transport.
Spinal-cord-patterned human spheroids allow sequential measurement of myelin damage, debris clearance, new oligodendrocytes and repair-associated myelination.
A molecular-descriptor classifier evaluated on an independently sourced set, with performance reported before and after confidence filtering.
Nine separate breast, ovarian and gastric cancer cell-line spheroid models used for comparative drug-response profiling.
Functional profiling of metastatic colorectal cancer spheroids against targeted agents and standard-of-care drug combinations.
A patient-specific iPSC-cardiomyocyte model comparing CAV3 localization and calcium transient behavior with a relative's control cells.
Two human 2H4 reporter-cell assays combine activation-related IL-2 signaling with information about antimitotic effects.
A human-cell BBB organoid assay estimates drug concentrations within single organoids from UPLC-MS/MS measurements of their lysates and compares uptake ratios with published in vivo brain-to-plasma data.
A hippocampal organoid study combines short valproic-acid exposure with mRNA and miRNA profiling and selected reporter assays.
A whole-body PBPK workflow evaluates a transporter-mediated drug interaction across age and ABCG2 genotype strata, then compares simulated rosuvastatin exposure with a monotherapy reference.
A human iPSC-derived multicellular liver-organoid comparison of LIPA-intact and LIPA-deficient tissue, followed by hepatocyte-directed restoration of LIPA expression.
Patient-derived colorectal organoid assembloids with stromal and immune cells used to compare recombinant IFNγ, an untargeted reference and Roche-engineered FAP-targeted IFNγ receptor agonists.
A coupled intestinal–cartilage chip to study gut-derived soluble signals, followed by separate metabolite tests in osteoarthritis-derived cartilage organoids.
Molecular-graph models for absorption, distribution, metabolism and excretion (ADME)-related properties were compared with measured metabolic stability, solubility and passive permeability in selected Tox21 compounds.
Structure, target-interaction and pathway fingerprints were compared on the same drug set; a majority-vote model was tested internally, on dissimilar compounds and on an independent data set.
Medium-matched imaging and growth-rate analysis compare drug responses after patient-derived tumor organoids stabilize in two culture conditions.
Lipid, function, expression and two CYP metabolite assays compare donor suspensions with three fatty-acid-induced human hepatic systems.
Autologous renal cell carcinoma (RCC) cells, cancer-associated fibroblasts (CAFs) and infiltrating immune cells are reconstituted to assess anti-PD-1 responses and perturb tumor–stromal–immune signaling.
Endothelin-1 (ET-1)-induced remodeling is assessed by gene expression, patch clamp and video-motion analysis to test selective responses to Cav1.3 C-terminal delivery.
Simultaneous voltage and calcium mapping compares hERG/electrolyte perturbation with focal injury in engineered human cardiomyocyte–fibroblast tissues.
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